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Phospho-Wee1 Antibody (pS123)

This gene encodes a nuclear protein, which is a tyrosine kinase belonging to the Ser/Thr family of protein kinases. This protein catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase.

Product Specifications

CAS Number

9007-83-4

Specifications

Dot blot: 1:500, IHC (Paraffin) : 1:50-1:100

UniProt

P30291

Host

Rabbit

Reactivity

Human

Immunogen

This phospho-Wee1 antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pS123 of human Wee1.

Clonality

Polyclonal

Isotype

Ig

Applications

DB, IHC, ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the phospho-Wee1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This phospho-Wee1 antibody is available for research use only.

Storage Conditions

Aliquot the phospho-Wee1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the phospho-Wee1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Dot blot analysis of nonphos (NP) and phospho-Wee1 antibody (P) . 50ng of phos-peptide or nonphos-peptide per dot were spotted.

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