P-STAT3 Antibody (pS727)

STAT3 is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. It mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunofluorescence: 1:200, Western blot: 1:1000, IHC (Paraffin) : 1:50-1:100

UniProt

P40763

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

This p-STAT3 antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pS727 of human STAT3.

Clonality

Polyclonal

Isotype

Applications

IF, WB, IHC, ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS pH 7.4 with 0.09% sodium azide

Reconstitution

Aliquot the p-STAT3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This phospho-p-STAT3 antibody is available for research use only.

Storage Conditions

Aliquot the p-STAT3 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the p-STAT3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of p-STAT3 antibody and mouse liver tissue/lysate collected before (-) or after (+) stimulation with IL-6 injection in mouse portal vein. Data is kindly provided by Drs. E. Bard-Chapeau and G-S. Feng from the Burnham Institute (La Jolla, CA) .