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Phospho-Src Antibody (pY215)

This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-Src kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene. Transcript Variant: This variant (1) represents the longer transcript. Both isoforms 1 and 2 encode the same protein.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000

UniProt

P12931

Host

Rabbit

Reactivity

Human

Immunogen

This phospho-Src antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pY215 of human Src.

Clonality

Polyclonal

Isotype

Ig

Applications

WB, ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the phospho-Src antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This phospho-Src antibody is available for research use only.

Storage Conditions

Aliquot the phospho-Src antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the phospho-Src antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Prediction Reactivity

Chicken, Mouse, Rat, Xenopus, Zebrafish

Image Legend

FG pancreatic carcinoma cell lines stably expressing vector alone (FG-V), the b3 integrin subunit (FG-b3), or a b3 truncation mutant (FG-759x) probed with phospho-Src antibody. Ab was diluted 1:500.

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