Phospho-SMAD4 Antibody (pT277)

Common mediator of signal transduction by TGF-beta (transforming growth factor) superfamily, SMAD4 is the common SMAD (co-SMAD) . It promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. It may act as a tumor suppressor.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunofluorescence: 1:10-1:50, Dot blot: 1:500, IHC (Paraffin) : 1:10-1:50

UniProt

Q13485

Host

Rabbit

Reactivity

Human

Immunogen

This phospho-SMAD4 antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pT277 of human SMAD4.

Clonality

Polyclonal

Isotype

Applications

IHC, IF, DB, ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the phospho-SMAD4 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This phospho-SMAD4 antibody is available for research use only.

Storage Conditions

Aliquot the phospho-SMAD4 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the phospho-SMAD4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Prediction Reactivity

Mouse, Rat

Image Legend

Confocal immunofluorescent analysis of phospho-SMAD4 antibody with HeLa cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green) . DAPI was used as a nuclear counterstain (blue) .