Phospho-p27Kip1 Antibody (pT157)
P27Kip1 is a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state.
Product Specifications
CAS Number
9007-83-4
Specifications
Dot blot: 1:500, IHC (Paraffin) : 1:50-1:100
UniProt
P46527
Host
Rabbit
Reactivity
Human
Immunogen
Clonality
Polyclonal
Isotype
Ig
Applications
DB, IHC, ELISA
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the phospho-p27Kip1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This phospho-p27Kip1 antibody is available for research use only.
Storage Conditions
Aliquot the phospho-p27Kip1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the phospho-p27Kip1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Dot blot analysis of phospho-p27Kip1 antibody. 50ng of phos-peptide or nonphos-peptide per dot were spotted.
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