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Phospho-HER4/ERBB4 Antibody (pY1188)

The HER4/ERBB4 gene is a member of the type I receptor tyrosine kinase subfamily that includes EGFR, ERBB2, and ERBB3. It encodes a receptor for NDF/heregulin.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000, IHC (Paraffin) : 1:50-1:100

UniProt

Q15303

Host

Rabbit

Reactivity

Human

Immunogen

This phospho-HER4 antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pY1188 of human HER4.

Clonality

Polyclonal

Isotype

Ig

Applications

WB, IHC, ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the phospho-HER4 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This phospho-HER4 antibody is available for research use only.

Storage Conditions

Aliquot the phospho-HER4 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the phospho-HER4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of phospho-HER4 antibody and FG pancreatic carcinoma cells treated with or without EGF (50ng/ml) for 15 min. Ab was used at 1:750. Data and protocol kindly provided by Dr. Weis of Cheresh Lab, UCSD.

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