Phospho-ERBB4 Antibody (pY1162)

HER4/ERBB4 is a member of the type I receptor tyrosine kinase subfamily that includes EGFR, ERBB2 and ERBB3. It is a receptor for NDF/heregulin.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000, IHC (Paraffin) : 1:50-1:100, Immunofluorescence: 1:10-1:50

UniProt

Q15303

Host

Rabbit

Reactivity

Human

Immunogen

This phospho-ERBB4 antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pY1162 of human ERBB4.

Clonality

Polyclonal

Isotype

Applications

WB, IHC, IF, ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the phospho-ERBB4 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This phospho-ERBB4 antibody is available for research use only.

Storage Conditions

Aliquot the phospho-ERBB4 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the phospho-ERBB4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Prediction Reactivity

Mouse, Rat

Image Legend

Western blot testing of phospho-ERBB4 antibody and FG pancreatic carcinoma cells treated with or without EGF (50ng/ml) for 15 min. Ab was used at 1:750. Data and protocol kindly provided by Dr. Weis of Cheresh Lab, UCSD.