Phospho-KDR Antibody / VEGFR2 (pY996)

KDR (FLK1) is a receptor for VEGF or VEGFC. This protein has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000, IHC (Paraffin) : 1:50-1:100

UniProt

P35968

Host

Rabbit

Reactivity

Human

Immunogen

This phospho-KDR antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pY996 of human KDR/FLK1.

Clonality

Polyclonal

Isotype

Applications

WB, IHC, ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the phospho-KDR antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This phospho-KDR antibody is available for research use only.

Storage Conditions

Aliquot the phospho-KDR antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the phospho-KDR antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic and cell surface

Image Legend

Phospho-KDR antibody used in western blot to detect phosphorylated KDR/FLK1 in HMVEC lysate. Endothelial cells were stimulated with 50ug/ml VEGF for 5min and Ab used at 1:500. Predicted molecular weight: ~152 (immature), 180-200 kDa (intermediate) and 220-230 kDa (mature) .