Phospho-CDC25A Antibody (pT507)

CDC25A is a member of the CDC25 family of phosphatases. CDC25A is required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated. Two transcript variants encoding different isoforms have been found for this gene.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunofluorescence: 1:25, Western blot: 1:250-1000, IHC (Paraffin) : 1:50-1:100

UniProt

P30304

Host

Rabbit

Reactivity

Human

Immunogen

This phospho-CDC25A antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pT507 of human CDC25A.

Clonality

Polyclonal

Isotype

Applications

WB, IHC, IF, ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the phospho-CDC25A antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This phospho-CDC25A antibody is available for research use only.

Storage Conditions

Aliquot the phospho-CDC25A antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the phospho-CDC25A antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Prediction Reactivity

Bovine, Mouse, Rat

Image Legend

Western blot analysis of extracts from HeLa cells, untreated or treated with Calyculin A, using phospho-Cdc25A antibody. Predicted molecular weight: 59-70 kDa.