PHGDH Antibody
3-Phosphoglycerate dehydrogenase (PHGDH) catalyzes the transition of 3-phosphoglycerate into 3-phosphohydroxypyruvate, which is the first and rate-limiting step in the phosphorylated pathway of serine biosynthesis, using NAD+/NADH as a cofactor.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1:1000, IHC (Paraffin) : 1:50-1:100, Flow Cytometry: 1:10-1:50
UniProt
O43175
Host
Rabbit
Reactivity
Human
Immunogen
A portion of amino acids 249-277 from the human protein was used as the immunogen for this PHGDH antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
WB, IHC, FACS, ELISA
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the PHGDH antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This PHGDH antibody is available for research use only.
Storage Conditions
Aliquot the PHGDH antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the PHGDH antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Prediction Reactivity
Mouse, Rat
Image Legend
PHGDH antibody western blot analysis in Ramos lysate. Predicted molecular weight ~56 kDa.
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