PHGDH Antibody

3-Phosphoglycerate dehydrogenase catalyzes the transition of 3-phosphoglycerate into 3-phosphohydroxypyruvate, which is the first and rate-limiting step in the phosphorylated pathway of serine biosynthesis, using NAD+/NADH as a cofactor.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000, IHC (Paraffin) : 1:50-1:100, Flow Cytometry: 1:10-1:50

UniProt

O43175

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

A portion of amino acids 140-168 from the human protein was used as the immunogen for this PHGDH antibody.

Clonality

Polyclonal

Isotype

Applications

WB, IHC, FACS, ELISA

Purity

Purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the PHGDH antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This PHGDH antibody is available for research use only.

Storage Conditions

Aliquot the PHGDH antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the PHGDH antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Prediction Reactivity

Bovine, Pig

Image Legend

Western blot analysis of PHGDH antibody and 293 cell lysate either nontransfected (Lane 1) or transiently transfected with the PHGDH gene (2) . Predicted molecular weight ~56 kDa.