NPM1 Antibody

Nucleophosmin/NPM1 is a ubiquitously expressed nucleolar protein that shuttles between the nucleus and cytoplasm. It is implicated in multiple functions, including ribosomal protein assembly and transport, control of centrosome duplication, and regulation of the tumor suppressor ARF. NPM1 mutations that relocalize NPM1 from the nucleus into the cytoplasm are associated with development of acute myeloid leukemia.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunofluorescence: 1:10-1:50, Western blot: 1:1000, Flow Cytometry: 1:10-1:50, IHC (Paraffin) : 1:10-1:50

UniProt

P06748

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 198-226 from the human protein was used as the immunogen for this NPM1 antibody.

Clonality

Polyclonal

Isotype

Applications

FACS, IHC, WB, IF, ELISA

Purity

Purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the NPM1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This NPM1 antibody is available for research use only.

Storage Conditions

Aliquot the NPM1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the NPM1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Fluorescent image of A549 cells stained with NPM1 antibody. Alexa Fluor 488 conjugated secondary was used (green) . NPM1 immunoreactivity is localized to the nucleus and nucleolus significantly.