Neuron Specific Enolase Antibody

NSE is one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha to gamma enolase occurs in neural tissue during development in rats and primates. [Wiki]

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000, IHC (Paraffin) : 1:50-1:100

UniProt

P09104

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 213-239 from human ENO2 was used as the immunogen for this Neuron Specific Enolase antibody.

Clonality

Polyclonal

Isotype

Applications

WB, IHC, ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Store at 4oC for up to one month. For long term, aliquot the Neuron Specific Enolase antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Neuron Specific Enolase antibody is available for research use only.

Storage Conditions

Store at 4°C for up to one month. For long term, aliquot the Neuron Specific Enolase antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the Neuron Specific Enolase antibody may be required due to differences in protocols and secondary/substrate sensitivity.