HNF4A Antibody
Transcriptionally controlled transcription factor. Binds to DNA sites required for the transcription of alpha 1-antitrypsin, apolipoprotein CIII, transthyretin genes and HNF1-alpha. May be essential for development of the liver, kidney and intestine. [UniProt]
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1:1000, IHC (Paraffin) : 1:10-1:50, Immunofluorescence: 1:10-1:50, Flow Cytometry: 1:10-1:50
UniProt
P41235
Host
Rabbit
Reactivity
Human, Rat
Immunogen
A portion of amino acids 281-312 from the human protein was used as the immunogen for this HNF4A antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
IF, FACS, WB, IHC, ELISA
Purity
Purified
Format
Purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the HNF4A antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This HNF4A antibody is available for research use only.
Storage Conditions
Aliquot the HNF4A antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the HNF4A antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Prediction Reactivity
Mouse
Location
Predominantly nuclear, present in cytoplasm in cells with high HNF4A expression
Image Legend
HNF4A antibody western blot analysis in 1) 293, 2) HepG2 cell line and 3) rat liver tissue lysate. Predicted molecular weight ~50 kDa.
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