Anti-LGR5 Antibody
LGR5/GPR49 is a receptor for R-spondins that potentiates the canonical Wnt signaling pathway and acts as a stem cell marker of the intestinal epithelium and the hair follicle. Upon binding to R-spondins (RSPO1, RSPO2, RSPO3 or RSPO4), associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G-protein coupled receptors, does not activate heterotrimeric G-proteins to transduce the signal. Involved in the development and/or maintenance of the adult intestinal stem cells during postembryonic development. [UniProt]
Product Specifications
CAS Number
9007-83-4
Specifications
IHC (Paraffin) : 1:10-1:50, Western blot: 1:1000
UniProt
O75473
Host
Rabbit
Reactivity
Human
Immunogen
A portion of amino acids 605-638 from the human protein was used as the immunogen for this anti-LGR5 antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
IHC, WB, ELISA
Purity
Purified
Format
Purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the anti-LGR5 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This anti-LGR5 antibody is available for research use only.
Storage Conditions
Aliquot the anti-LGR5 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the anti-LGR5 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Anti-LGR5 antibody western blot analysis in HL-60 lysate
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