ALDOA Antibody

Aldolase A (fructose-bisphosphate aldolase) is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Three aldolase isozymes (A, B, and C), encoded by three different genes, are differentially expressed during development. Aldolase A is found in the developing embryo and is produced in even greater amounts in adult muscle. Aldolase A expression is repressed in adult liver, kidney and intestine and similar to aldolase C levels in brain and other nervous tissue. Aldolase A deficiency has been associated with myopathy and hemolytic anemia.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000, IHC (Paraffin) : 1:10-1:50

UniProt

P04075

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

A portion of amino acids 303-331 from the human protein was used as the immunogen for this ALDOA antibody.

Clonality

Polyclonal

Isotype

Applications

WB, IHC, ELISA

Purity

Purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the ALDOA antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This ALDOA antibody is available for research use only.

Storage Conditions

Aliquot the ALDOA antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the ALDOA antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Prediction Reactivity

Rabbit

Image Legend

Western blot analysis of lysate from 1) MCF-7, 2) HeLa cell line and 3) human skeletal muscle tissue lysate using ALDOA antibody at 1:1000. Predicted molecular weight ~40 kDa.