KLF4 Antibody

Transcription factor; can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development Contributes to the down-regulation of p53/TP53 transcription.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000, Immunofluorescence: 1:10-1:50, IHC (Paraffin) : 1:10-1:50

UniProt

O43474

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

A portion of amino acids 69-101 from the human protein was used as the immunogen for this KLF4 antibody.

Clonality

Polyclonal

Isotype

Applications

WB, IF, IHC, ELISA

Purity

Purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Store at 4oC for up to one month. For long term, aliquot the KLF4 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This KLF4 antibody is available for research use only.

Storage Conditions

Store at 4°C for up to one month. For long term, aliquot the KLF4 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the KLF4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.