IKBa Antibody
NFKB1 or NFKB2 is bound to REL, RELA, or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine) .
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1:1000
UniProt
P25963
Host
Rabbit
Reactivity
Human
Immunogen
A portion of amino acids 1-30 from the human protein was used as the immunogen for this IKBa antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
WB, ELISA
Purity
Purified
Format
Purified
Buffer
In 1X PBS pH 7.4 with 0.09% sodium azide
Reconstitution
Aliquot the IKBa antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This IKBa antibody is available for research use only.
Storage Conditions
Aliquot the IKBa antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the IKBa antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
IKBa antibody used in western blot to detect IKB-alpha in Jurkat cell lysate
Frequently Asked Questions
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