P62 Antibody
SQSTM1/p62 is an adapter protein which binds ubiquitin and may regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1. This protein may play a role in titin/TTN downstream signaling in muscle cells, and may also regulate signaling cascades through ubiquitination. This protein is involved in cell differentiation, apoptosis, immune response and regulation of K (+) channels. SQSTM1/p62 also appears to play a role in macroautophagic removal of intracellular protein aggregates. Cellular depletion studies of SQSTM1/p62 have indicated a role for association with LC3 and aggregate proteins in order to facilitate normal formation of the autophagosome.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1:1000, IHC (Paraffin) : 1:50-1:100, Immunofluorescence: 1:50-1:100
UniProt
Q13501
Host
Rabbit
Reactivity
Human
Immunogen
A portion of amino acids 317-346 from the human protein was used as the immunogen for this p62 antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
WB, IF, IHC, ELISA
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
In 1X PBS pH 7.4 with 0.09% sodium azide
Reconstitution
Aliquot the p62 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This p62 antibody is available for research use only.
Storage Conditions
Aliquot the p62 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the p62 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Prediction Reactivity
Mouse, Rat
Image Legend
Western blot testing of p62 antibody at 1:500 dilution + HeLa lysate; Predicted band size : 47~62 kDa.
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