Mib1 Antibody
MIB is an E3 ubiquitin-protein ligase that mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. This protein positively regulates the Delta-mediated Notch signaling by ubiquitinating the intracellular domain of Delta, leading to endocytosis of Delta receptors. MIB probably mediates ubiquitination and subsequent proteasomal degradation of DAPK1, thereby antagonizing anti-apoptotic effects of DAPK1 to promote TNF-induced apoptosis.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1:1000, Immunofluorescence: 1:20-1:100, IHC (Paraffin) : 1:50-1:100
UniProt
Q86YT6
Host
Rabbit
Reactivity
Human
Immunogen
A portion of amino acids 13-42 from the human protein was used as the immunogen for this Mib1 antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
IF, IHC, WB, ELISA
Purity
Purified
Format
Purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the Mib1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This Mib1 antibody is available for research use only.
Storage Conditions
Aliquot the Mib1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the Mib1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Prediction Reactivity
Mouse, Zebrafish, Xenopus
Image Legend
Mib1 antibody western blot analysis of human K562 lysate. Predicted molecular weight ~110 kDa.
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