PGP 9.5 Antibody

UCHL1 is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. Expression of UCHL1 is highly specific to neurons and to cells of the diffuse neuroendocrine system and their tumors. It is present in all neurons.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000, Immunofluorescence: 1:10-1:50, IHC (Paraffin) : 1:10-1:50

UniProt

P09936

Host

Rabbit

Reactivity

Human, Rat

Immunogen

A portion of amino acids 187-214 from the human protein was used as the immunogen for this PGP 9.5 antibody.

Clonality

Polyclonal

Isotype

Applications

IHC, IF, WB, ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the PGP 9.5 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This PGP 9.5 antibody is available for research use only.

Storage Conditions

Aliquot the PGP 9.5 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the PGP 9.5 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Prediction Reactivity

Bovine, Mouse, Pig

Image Legend

PGP 9.5 antibody western blot analysis in (1) U266, (2) NCI-H1299, (3) mouse Neuro-2a cell line and (4) mouse brain tissue lysate. Predicted molecular weight ~25 kDa.