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PGP9.5 Antibody

PGP9.5 is a Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity. [UniProt]

Product Specifications

Specifications

Western blot: 1:1000, IHC (Paraffin) : 1:10-1:50, Immunofluorescence: 1:10-1:50, Flow Cytometry: 1:10-1:50

UniProt

P09936

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

A portion of amino acids 16-46 from the human protein was used as the immunogen for this PGP9.5 antibody.

Clonality

Polyclonal

Isotype

Ig

Applications

WB, IHC, IF, FACS, ELISA

Purity

Purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the PGP9.5 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This PGP9.5 antibody is available for research use only.

Storage Conditions

Aliquot the PGP9.5 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Product Datasheet

https://www.nsjbio.com/tds-pdf/pgp95-antibody-f47951

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the PGP9.5 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Prediction Reactivity

Bovine, Pig

CAS Number

9007-83-4

Image Legend

IHC analysis of FFPE human brain tissue stained with PGP9.5 antibody.

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