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Oct-4 Antibody / POU5F1

Transcription factors containing the POU homeodomain have been shown to be important regulators of tissue-specific gene expression in lymphoid and pituitary differentiation and in early mammalian development.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000, Immunofluorescence: 1:10-1:50

UniProt

Q01860

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 110-141 from the human protein was used as the immunogen for this Oct-4 antibody.

Clonality

Polyclonal

Isotype

Ig

Applications

WB, IF, ELISA

Purity

Purified

Format

Purified

Buffer

In 1X PBS pH 7.4 with 0.09% sodium azide

Reconstitution

Store at 4oC for up to one month. For long term, aliquot the Oct-4 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Oct-4 antibody is available for research use only.

Storage Conditions

Store at 4°C for up to one month. For long term, aliquot the Oct-4 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the Oct-4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot analysis of Oct-4 antibody and HL-60 lysate. Predicted molecular weight ~38/30kDa (isoform A/B), commonly observed at 38-45 kDa.

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