SOX4 Antibody

SOX4 is a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The protein may act as a transcriptional regulator after forming a protein complex with other proteins, such as syndecan binding protein (syntenin) . The protein may function in the apoptosis pathway leading to cell death as well as to tumorigenesis and may mediate downstream effects of parathyroid hormone (PTH) and PTH-related protein (PTHrP) in bone development. The solution structure has been resolved for the HMG-box of a similar mouse protein.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000, IHC (Paraffin) : 1:50-1:100, Flow Cytometry: 1:10-1:50, Immunofluorescence: 1:10-1:50

UniProt

Q06945

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 86-114 from the human protein was used as the immunogen for this SOX4 antibody.

Clonality

Polyclonal

Isotype

Applications

IF, WB, IHC, FACS, ELISA

Purity

Purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the SOX4 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This SOX4 antibody is available for research use only.

Storage Conditions

Aliquot the SOX4 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the SOX4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Nuclear, cytoplasmic

Image Legend

Fluorescent confocal image of HeLa cells stained with SOX4 antibody. Alexa Fluor 488 conjugated secondary (green) was used. SOX4 immunoreactivity is localized to mitochondria strongly and nucleus weakly.