PROX1 Antibody / Prospero homeobox 1
The expression pattern of Prox1 suggests that it has a role in a variety of embryonic tissues, including lens. Prox mRNA is present in many different human tissues with lens demonstrating the highest level. Homozygous Prox1-null mice die at midgestation from multiple developmental defects, and a targeted effect on lens development has been reported. Prox1 inactivation caused abnormal cellular proliferation, downregulated expression of the cell cycle inhibitors Cdkn1b and Cdkn1c, misexpression of E-cadherin, and excessive apoptosis. Consequently, mutant lens cells failed to polarize and elongate properly, resulting in a hollow lens.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1:500, Immunofluorescence: 1:10-1:50
UniProt
Q92786
Host
Rabbit
Reactivity
Human, Rat
Immunogen
A portion of amino acids 492-522 from the human protein was used as the immunogen for this PROX1 antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
WB, IF, ELISA
Purity
Purified
Format
Purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the PROX1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This PROX1 antibody is available for research use only.
Storage Conditions
Aliquot the PROX1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the PROX1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Prediction Reactivity
Mouse
Image Legend
Fluorescent confocal image of A2058 cell stained with PROX1 antibody at 1:25. PROX1 immunoreactivity is localized to the nucleus.
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