PDX1 Antibody / Pyruvate dehydrogenase protein X / PDXH
PDX1/PDHX, located in the mitochondrial matrix, is required for anchoring dihydrolipoamide dehydrogenase (E3) to the dihydrolipoamide transacetylase (E2) core of the pyruvate dehydrogenase complexes of eukaryotes. This specific binding is essential for a functional PDH complex. Eukaryotic pyruvate dehydrogenase complexes are organized about a core consisting of the oligomeric dihydrolipoamide acetyl-transferase, around which are arranged multiple copies of pyruvate dehydrogenase, dihydrolipoamide dehydrogenase and protein X bound by noncovalent bonds. Defects in PDHX are a cause of lacticacidemia. PDX1 belongs to the 2-oxoacid dehydrogenase family and contains 1 lipoyl-binding domain.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1:1000
UniProt
O00330
Host
Rabbit
Reactivity
Human
Immunogen
A portion of amino acids 429-459 from the human protein was used as the immunogen for this PDX1 antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
WB, ELISA
Purity
Purified
Format
Purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the PDX1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This PDX1 antibody is available for research use only.
Storage Conditions
Aliquot the PDX1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the PDX1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Western blot analysis of lysate from WiDr cell line using PDX1 at 1:1000. Predicted molecular weight ~54kDa.
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