HIF1A Antibody
Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT) . This gene encodes the alpha subunit of HIF-1. Overexpression of a natural antisense transcript (aHIF) of this gene has been shown to be associated with nonpapillary renal carcinomas. Two alternative transcripts encoding different isoforms have been identified.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1:1000
UniProt
Q16665
Host
Rabbit
Reactivity
Human
Immunogen
A portion of amino acids 735-762 from the human protein was used as the immunogen for this HIF1A antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
WB, ELISA
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
In 1X PBS pH 7.4 with 0.09% sodium azide
Reconstitution
Aliquot the HIF1A antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This HIF1A antibody is available for research use only.
Storage Conditions
Aliquot the HIF1A antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the HIF1A antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Prediction Reactivity
Bovine
Location
Nuclear, possible cytoplasmic
Image Legend
HIF1A antibody western blot analysis in A549 lysate. Routinely observed molecular weight: 100~120 kDa.
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