Epidermal Growth Factor Receptor Antibody

The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. Multiple alternatively spliced transcript variants that encode different protein isoforms have been found for this gene.

Product Specifications

CAS Number

9007-83-4

Specifications

IHC (Paraffin) : 1:25, Western blot: 1:1000

UniProt

P00533

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 259-287 from the human protein was used as the immunogen for this Epidermal Growth Factor Receptor antibody.

Clonality

Polyclonal

Isotype

Applications

IHC, WB, ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the Epidermal Growth Factor Receptor antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Epidermal Growth Factor Receptor antibody is available for research use only.

Storage Conditions

Aliquot the Epidermal Growth Factor Receptor antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the Epidermal Growth Factor Receptor antibody may be required due to differences in protocols and secondary/substrate sensitivity.