P65 Antibody
NFKB1 or NFKB2 is bound to REL, RELA, or RELB to form the NFKB complex. The p50 (NFKB1) /p65 (RELA) heterodimer is the most abundant form of NFKB. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivates NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NFKB complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs. [OMIM]
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1:1000
UniProt
Q04206
Host
Rabbit
Reactivity
Human
Immunogen
A portion of amino acids 32-60 from the human protein was used as the immunogen for this p65 antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
WB, ELISA
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the p65 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This p65 antibody is available for research use only.
Storage Conditions
Aliquot the p65 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the p65 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Prediction Reactivity
Mouse
Image Legend
Western blot analysis of (1) HL-60 and (2) U251 MG cell line using p65 antibody at 1:1000.
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