Bcl-2 Antibody

Bcl-2 suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1) . [UniProt]

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000

UniProt

P10415

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 144-171 from the human protein was used as the immunogen for this Bcl-2 antibody.

Clonality

Polyclonal

Isotype

Applications

WB, ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS pH 7.4 with 0.09% sodium azide

Reconstitution

Can be stored at 4oC for one month. For long term, aliquot the Bcl-2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Bcl-2 antibody is available for research use only.

Storage Conditions

Can be stored at 4°C for one month. For long term, aliquot the Bcl-2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the Bcl-2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.