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EIF4E2 Antibody

EIF4E2 is expressed exclusively in the cytoplasm. This protein recognizes and binds the 7 methylguanosine containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000, IHC (Paraffin) : 1:50-1:100

UniProt

O60573

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 12-41 from the human protein was used as the immunogen for this EIF4E2 antibody.

Clonality

Polyclonal

Isotype

Ig

Applications

WB, IHC, ELISA

Purity

Purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Store at 4oC for up to one month. For long term, aliquot the EIF4E2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This EIF4E2 antibody is available for research use only.

Storage Conditions

Store at 4°C for up to one month. For long term, aliquot the EIF4E2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the EIF4E2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

IHC analysis of formalin fixed paraffin embedded human breast carcinoma stained with EIF4E2 antibody

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