EIF4E Antibody

EIF4E is a eukaryotic translation initiation factor involved in directing ribosomes to the cap structure of mRNAs. It is a 27-kD polypeptide that exists as both a free form and as part of a multiprotein complex termed EIF4F. The EIF4E polypeptide is the rate-limiting component of the eukaryotic translation apparatus and is involved in the mRNA-ribosome binding step of eukaryotic protein synthesis. The other subunits of EIF4F are a 50-kD polypeptide, termed EIF4A (see MIM 601102), that possesses ATPase and RNA helicase activities, and a 220-kD polypeptide, EIF4G.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000, IHC (Paraffin) : 1:50-1:100, Flow Cytometry: 1:10-1:50

UniProt

P06730

Host

Rabbit

Reactivity

Human

Immunogen

This EIF4E antibody was produced from rabbits immunized with a recombinant protein from human VGFR2.

Clonality

Polyclonal

Isotype

Applications

FACS, IHC, WB, ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the EIF4E antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This EIF4E antibody is available for research use only.

Storage Conditions

Aliquot the EIF4E antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the EIF4E antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

EIF4E antibody flow cytometric analysis of HL-60 cells (bottom histogram) compared to a negative control (top histogram) . FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.