Beclin Antibody
Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole) . Beclin 1 plays a role in two fundamentally important cell biological pathways: autophagy and apoptosis. Beclin 1 is thought to function as a VPS and autophagy protein as part of a complex with Class III PI3 kinase, Vps34.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1:1000, IHC (Paraffin) : 1:50-1:100, Flow Cytometry: 1:10-1:50
UniProt
Q14457
Host
Rabbit
Reactivity
Human, Mouse
Immunogen
A portion of amino acids 210-239 from the human protein was used as the immunogen for this Beclin antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
FACS, WB, IHC, ELISA
Purity
Purified
Format
Purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the Beclin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This Beclin antibody is available for research use only.
Storage Conditions
Aliquot the Beclin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the Beclin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Prediction Reactivity
Bovine, Pig, Rat
Image Legend
Western blot testing of Beclin antibody and (1) A2058, (2) HeLa, (3) mouse brain, (4) Y79, and (5) HL-60 lysate
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