ATG7 Antibody

Autophagy is a process of bulk degradation of cytoplasmic components by the lysosomal/vacuolar system. ATG7 is a ubiquitin-activating enzyme E1-like protein essential for the Apg12 conjugation system that mediates membrane fusion in autophagy. [OMIM]

Product Specifications

CAS Number

9007-83-4

Specifications

Immunofluorescence: 1:200, Western blot: 1:1000, IHC (Paraffin) : 1:50-1:100

UniProt

O95352

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

A portion of amino acids 494-523 from the human protein was used as the immunogen for this ATG7 antibody.

Clonality

Polyclonal

Isotype

Applications

IF, WB, IHC, ELISA

Purity

Purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the ATG7 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This ATG7 antibody is available for research use only.

Storage Conditions

Aliquot the ATG7 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the ATG7 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Prediction Reactivity

Rat

Image Legend

Fluorescent image of Chloroquine treated U251 cells stained with ATG7 antibody at 1:200. Immunoreactivity is localized to autophagic vacuoles in the cytoplasm.