HMGB-1 Antibody
DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V (D) J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS) . Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells (By similarity) . [UniProt]
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1:1000
UniProt
P09429
Host
Rabbit
Reactivity
Human
Immunogen
A portion of amino acids 152-180 from the human protein was used as the immunogen for this HMGB-1 antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
WB, ELISA
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
In 1X PBS pH 7.4 with 0.09% sodium azide
Reconstitution
Aliquot the HMGB-1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This HMGB-1 antibody is available for research use only.
Storage Conditions
Aliquot the HMGB-1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the HMGB-1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Prediction Reactivity
Mouse, Rat, Bovine, Pig, Hamster
Image Legend
HMGB-1 antibody western blot analysis in HeLa lysate. Predicted molecular weight ~25 kDa.
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