KRAS Antibody

This gene, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:500-1:2000, Immunofluorescence: 1:10-1:50, Flow Cytometry: 1:10-1:50

UniProt

P01116

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A portion of amino acids 146-174 from the human protein was used as the immunogen for this KRAS antibody.

Clonality

Polyclonal

Isotype

Applications

WB, IF, FACS, ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the KRAS antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This KRAS antibody is available for research use only.

Storage Conditions

Aliquot the KRAS antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the KRAS antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of 1) human HeLa, 2) human 293/T17, 3) mouse C2C12, 4) rat C6, 5) human HT-29 and 6) rat PC-12 cell lysate with KRAS antibody at 1:500. Expected molecular weight: 20-25 kDa.