Connexin 43 Antibody

Gap junction protein, alpha 1 is a member of the connexin gene family and a component of gap junctions. Gap junctions are composed of arrays of intercellular channels and provide a route for the diffusion of materials of low molecular weight from cell to cell. Connexin 43 is the major protein of gap junctions in the heart, and gap junctions are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. Connexin 43 is targeted by several protein kinases that regulate myocardial cell-cell coupling. A related intron-less connexin 43 pseudogene, GJA1P, has been mapped to chromosome 5.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000, IHC (Paraffin) : 1:50-1:100

UniProt

P17302

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A portion of amino acids 107-138 from the human protein was used as the immunogen for this Connexin 43 antibody.

Clonality

Polyclonal

Isotype

Applications

WB, IHC, ELISA

Purity

Purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the Connexin 43 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Connexin 43 antibody is available for research use only.

Storage Conditions

Aliquot the Connexin 43 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the Connexin 43 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Prediction Reactivity

Bovine, Xenopus

Image Legend

Western blot analysis of lysate from (1) HeLa, (2) U-87 MG, (3) rat C6 cell line and (4) mouse heart tissue lysate using Connexin 43 antibody at 1:1000. Predicted molecular weight: 43 kDa