TLR6 Antibody

TLR6, a Type I membrane protein that belongs to the Toll-like rceptor family, participates in the innate immune response to Gram-positive bacteria and fungi. It acts via MyD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. The protein recognizes mycoplasmal macrophage-activating lipopeptide-2kD (MALP-2), soluble tuberculosis factor (STF), phenol-soluble modulin (PSM) and B.burgdorferi outer surface protein A lipoprotein (OspA-L) cooperatively with TLR2. It binds to TLR2 via their respective extracellular domains, and to MyD88 via their respective TIR domains. TLR6 is detected in monocytes, CD11c+ immature dendritic cells, plasmacytoid pre-dendritic cells and dermal microvessel endothelial cells.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow Cytometry: 1:10-1:50, Western blot: 1:1000, IHC (Paraffin) : 1:50-1:100

UniProt

Q9Y2C9

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 393-423 from the human protein was used as the immunogen for this TLR6 antibody.

Clonality

Polyclonal

Isotype

Applications

FACS, WB, IHC, ELISA

Purity

Purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the TLR6 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This TLR6 antibody is available for research use only.

Storage Conditions

Aliquot the TLR6 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the TLR6 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Flow cytometric analysis of NCI-H460 cells using TLR6 antibody (bottom histogram) compared to a negative control (top histogram) . FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.