TLR5 Antibody
TLR5, a Type I membrane protein belonging to the Toll-like receptor family, participates in the innate immune response to microbial agents. It also plays a role in mediating detection of bacterial flagellins. TLR5 acts via MyD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. This protein binds to TIRAP and MyD88 via their respective TIR domains TLR5 is highly expressed in liver, and is detected in lung and at very low levels in most other tissues. The TLR5 gene lies in a locus that is associated with susceptibility to Salmonella. Inbred strains of mice can be classified into 3 categories according to their resistance to infection with S.typhimurium: susceptible (BALB/c, C57BL/6, C3H/He), intermediate (DBA/2, C75L) and resistant (A, CBA) . The strain MOLF/Ei is highly susceptible to the infection, has an unique TLR5 haplotype and a lower expression of TRL5.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1:1000, IHC (Paraffin) : 1:50-1:100
UniProt
Q9JLF7
Host
Rabbit
Reactivity
Human, Mouse
Immunogen
A portion of amino acids 825-856 from the mouse protein was used as the immunogen for this TLR5 antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
WB, IHC, ELISA
Purity
Purified
Format
Purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the TLR5 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This TLR5 antibody is available for research use only.
Storage Conditions
Aliquot the TLR5 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the TLR5 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Location
Cytoplasmic, membrane
Image Legend
IHC analysis of FFPE human hepatocarcinoma tissue stained with the TLR5 antibody
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