Anti-TLR4 Antibody
TLR4, a type I membrane protein that belongs to the Toll-like receptor family, cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS) . It acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response TLR4 Belongs to the lipopolysaccharide (LPS) receptor, a multi-protein complex containing at least CD14, LY96 and TLR. TLR4 binds to LY96 via the extracellular domain, and to MyD88 and TIRAP via their respective TIR domains. The protein contains 19 leucine-rich (LRR) repeats, and It is highly expressed in heart, spleen, lung and muscle. Lower levels are found in liver and kidney. Interstrain analyses reveal that TLR4 is a polymorphic protein and that the extracellular domain is far more variable than the cytoplasmic domain, which is variable at the C-terminal.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1:1000
UniProt
Q9QUK6
Host
Rabbit
Reactivity
Mouse
Immunogen
A portion of amino acids 25-55 from the mouse protein was used as the immunogen for this anti-TLR4 antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
WB, ELISA
Purity
Purified
Format
Purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the anti-TLR4 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This anti-TLR4 antibody is available for research use only.
Storage Conditions
Aliquot the anti-TLR4 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the anti-TLR4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Western blot analysis of anti-TLR4 antibody and mouse spleen cell lysate
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