CD19 Antibody
Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. CD19 is a cell surface molecule which assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1:500-100, Immunofluorescence: 1:10-1:50, Flow Cytometry: 1:10-1:50, IHC (Paraffin) : 1:10-1:50
UniProt
P15391
Host
Rabbit
Reactivity
Human
Immunogen
A portion of amino acids 143-172 from the human protein was used as the immunogen for this CD19 antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
WB, IF, FACS, IHC, ELISA
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
In 1X PBS pH 7.4 with 0.09% sodium azide
Reconstitution
Aliquot the CD19 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This CD19 antibody is available for research use only.
Storage Conditions
Aliquot the CD19 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the CD19 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Location
Cell surface, cytoplasmic
Image Legend
Western blot analysis of lysate from Ramos cell line using CD19 antibody at 1:500. Expected size is 60~100 kDa depending on glycosylation level.
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