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CD19 Antibody

Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. CD19 is a cell surface molecule which assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:500-100, Immunofluorescence: 1:10-1:50, Flow Cytometry: 1:10-1:50, IHC (Paraffin) : 1:10-1:50

UniProt

P15391

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 143-172 from the human protein was used as the immunogen for this CD19 antibody.

Clonality

Polyclonal

Isotype

Ig

Applications

WB, IF, FACS, IHC, ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS pH 7.4 with 0.09% sodium azide

Reconstitution

Aliquot the CD19 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This CD19 antibody is available for research use only.

Storage Conditions

Aliquot the CD19 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the CD19 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cell surface, cytoplasmic

Image Legend

Western blot analysis of lysate from Ramos cell line using CD19 antibody at 1:500. Expected size is 60~100 kDa depending on glycosylation level.

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