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Placental Alkaline Phosphatase Antibody

There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific) . The first three are located together on chromosome 2 while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme, also referred to as the heat stable form, that is expressed primarily in the placenta although it is closely related to the intestinal form of the enzyme as well as to the placental-like form. The coding sequence for this form of alkaline phosphatase is unique in that the 3' untranslated region contains multiple copies of an Alu family repeat. In addition, this gene is polymorphic and three common alleles (type 1, type 2 and type 3) for this form of alkaline phosphatase have been well characterized.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000, IHC (Paraffin) : 1:10-1:50

UniProt

P05187

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 56-83 from the human protein was used as the immunogen for this placental Alkaline Phosphatase antibody.

Clonality

Polyclonal

Isotype

Ig

Applications

WB, IHC, ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the placental Alkaline Phosphatase antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This placental Alkaline Phosphatase antibody is available for research use only.

Storage Conditions

Aliquot the placental Alkaline Phosphatase antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the placental Alkaline Phosphatase antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Placental Alkaline Phosphatase antibody western blot analysis in HepG2 lysate

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