NANOG Antibody
NANOG protein is a 305 amino acid protein with a conserved homeodomain motif that is localized to the nuclear component of cells. The homeodomain region facilitates DNA binding. It is a transcription factor in embryonic stem cells (ESCs) and is thought to be a key factor in maintaining pluripotency. NANOG is thought to function in concert with other factors such as POU5F1 (Oct-4) and SOX2 to establish ESC identity. [Wiki]
Product Specifications
CAS Number
9007-83-4
Specifications
IHC (Paraffin) : 1:10-1:50, Immunofluorescence: 1:10-1:50, Flow Cytometry: 1:10-1:50
UniProt
Q9H9S0
Host
Rabbit
Reactivity
Human
Immunogen
A portion of amino acids 94-123 from the human protein was used as the immunogen for this NANOG antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
IF, FACS, IHC, ELISA
Purity
Purified
Format
Purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the NANOG antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This NANOG antibody is available for research use only.
Storage Conditions
Aliquot the NANOG antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the NANOG antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Prediction Reactivity
Bovine
Image Legend
IHC analysis of FFPE human testis tissue stained with NANOG antibody
Frequently Asked Questions
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