NANOG Antibody (N-Terminal Region)
NANOG is a transcription regulator involved in inner cell mass and embryonic stem (ES) cell proliferation and self-renewal. It imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. This protein blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. NANOG acts as a transcriptional activator or repressor. It binds optimally to the DNA consensus sequence 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. When overexpressed, this protein promotes cells to enter into S phase and proliferation. [UniProt]
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1:1000, IHC (Paraffin) : 1:10-1:50, Flow Cytometry: 1:10-1:50, Immunofluorescence: 1:10-1:50
UniProt
Q9H9S0
Host
Rabbit
Reactivity
Human
Immunogen
A portion of amino acids 15-49 from the human protein was used as the immunogen for this NANOG antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
WB, IF, IHC, FACS, ELISA
Purity
Purified
Format
Purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the NANOG antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This NANOG antibody is available for research use only.
Storage Conditions
Aliquot the NANOG antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the NANOG antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Prediction Reactivity
Primate
Image Legend
Western blot analysis of NANOG antibody and K562 lysate; Predicted molecular weight: 35-45 kDa.
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