LIN28B Antibody
Lin-28 homolog B (LIN28B) is overexpressed in hepatocellular carcinoma. The heterochronic gene lin-28 is a key regulator of developmental timing in the nematode Caenorhabditis elegans. Similar with lin-28 proteins, LIN28B conserves a cold shock domain and a pair of CCHC zinc finger domains. Phylogenetic analysis suggests that they might arise as a result of duplication from an ancestral gene. Overexpression of LIN28B was noted in most HCC cell lines and clinical samples. A short LIN28B isoform was also identified in non-tumor liver tissue and fetal liver. Although predominantly localized in the cytoplasm, LIN28B protein shows cell cycle-dependent nuclear translocation in Huh7 cells. Induced expression of exogenous LIN28B in a tet-off cell line promoted cancer cell proliferation.
Product Specifications
CAS Number
9007-83-4
Specifications
Immunofluorescence: 1:100, Western blot: 1:1000, IHC (Paraffin) : 1:10-1:50, Flow Cytometry: 1:10-1:50
UniProt
Q6ZN17
Host
Rabbit
Reactivity
Human
Immunogen
A portion of amino acids 1-30 from the human protein was used as the immunogen for this LIN28B antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
IF, WB, IHC, FACS, ELISA
Purity
Purified
Format
Purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the LIN28B antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This LIN28B antibody is available for research use only.
Storage Conditions
Aliquot the LIN28B antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the LIN28B antibody may be required due to differences in protocols and secondary/substrate sensitivity.
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