LIN28B Antibody

Lin-28 homolog B (LIN28B) is overexpressed in hepatocellular carcinoma. The heterochronic gene lin-28 is a key regulator of developmental timing in the nematode Caenorhabditis elegans. Similar with lin-28 proteins, LIN28B conserves a cold shock domain and a pair of CCHC zinc finger domains. Phylogenetic analysis suggests that they might arise as a result of duplication from an ancestral gene. Overexpression of LIN28B was noted in most HCC cell lines and clinical samples. A short LIN28B isoform was also identified in non-tumor liver tissue and fetal liver. Although predominantly localized in the cytoplasm, LIN28B protein shows cell cycle-dependent nuclear translocation in Huh7 cells. Induced expression of exogenous LIN28B in a tet-off cell line promoted cancer cell proliferation.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunofluorescence: 1:100, Western blot: 1:1000, IHC (Paraffin) : 1:10-1:50, Flow Cytometry: 1:10-1:50

UniProt

Q6ZN17

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 1-30 from the human protein was used as the immunogen for this LIN28B antibody.

Clonality

Polyclonal

Isotype

Applications

IF, WB, IHC, FACS, ELISA

Purity

Purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the LIN28B antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This LIN28B antibody is available for research use only.

Storage Conditions

Aliquot the LIN28B antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the LIN28B antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Fluorescent confocal image of SY5Y cells stained with LIN28B antibody. Alexa Fluor 488 conjugated secondary (green) was used (1:1000, 1h) . Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min) . LIN28B immunosignal is localized predominantly to the cytoplasm.