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Anti-E Cadherin Antibody

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. E Cadherin is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7. [UniProt]

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000, Flow Cytometry: 1:10-1:50

UniProt

P12830

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 833-862 from the human protein was used as the immunogen for this anti-E Cadherin antibody.

Clonality

Polyclonal

Isotype

Ig

Applications

WB, FACS, ELISA

Purity

Purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the anti-E Cadherin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This anti-E Cadherin antibody is available for research use only.

Storage Conditions

Aliquot the anti-E Cadherin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the anti-E Cadherin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic, membranous

Image Legend

Western blot analysis of anti-E Cadherin antibody and A375 lysate. Expected molecular weight: 135 kDa (precursor), 80-120 kDa (mature, depending on gylcosylation level) .

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