E Cadherin Antibody

CDH1 is a classical cadherin from the cadherin superfamily. This protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000, Flow Cytometry: 1:10-1:50

UniProt

P12830

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 160-189 from the human protein was used as the immunogen for this E Cadherin antibody.

Clonality

Polyclonal

Isotype

Applications

WB, FACS, ELISA

Purity

Purified

Format

Purified

Buffer

In 1X PBS pH 7.4 with 0.09% sodium azide

Reconstitution

Aliquot the E Cadherin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This E Cadherin antibody is available for research use only.

Storage Conditions

Aliquot the E Cadherin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the E Cadherin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic, membranous

Image Legend

Western blot analysis of E Cadherin antibody and 293 lysate. Expected molecular weight: 135 kDa (precursor), 80-120 kDa (mature, depending on gylcosylation level) .