RUNX3 Antibody

This gene encodes a member of the runt domain-containing family of transcription factors. A heterodimer of this protein and a beta subunit forms a complex that binds to the core DNA sequence 5'-PYGPYGGT-3' found in a number of enhancers and promoters, and can either activate or suppress transcription. It also interacts with other transcription factors. It functions as a tumor suppressor, and the gene is frequently deleted or transcriptionally silenced in cancer. Multiple transcript variants encoding different isoforms have been found for this gene.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000, IHC (Paraffin) : 1:10-1:50, Immunofluorescence: 1:10-1:50

UniProt

Q13761

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 168-197 from the human protein was used as the immunogen for this RUNX3 antibody.

Clonality

Polyclonal

Isotype

Applications

IHC, IF, WB, ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the RUNX3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This RUNX3 antibody is available for research use only.

Storage Conditions

Aliquot the RUNX3 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the RUNX3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot analysis of lysate from 1) HL-60 and 2) Jurkat cell line using RUNX3 antibody at 1:1000. Predicted molecular weight: ~44 kDa, isoforms can be observed at 42-48 kDa.