ALDH1A1 Antibody
ALDH1A1 belongs to the aldehyde dehydrogenases family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of this enzyme, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Orientals have only the cytosolic isozyme, missing the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Orientals than among Caucasians could be related to the absence of the mitochondrial isozyme.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1:1000, Immunofluorescence: 1:100, IHC (Paraffin) : 1:50-1:100, Flow Cytometry: 1:10-1:50
UniProt
P00352
Host
Rabbit
Reactivity
Human
Immunogen
This ALDH1A1 antibody was produced from rabbits immunized with human ALDH1A1 recombinant protein.
Clonality
Polyclonal
Isotype
Ig
Applications
IF, IHC, FACS, WB, ELISA
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the ALDH1A1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This ALDH1A1 antibody is available for research use only.
Storage Conditions
Aliquot the ALDH1A1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the ALDH1A1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Western blot analysis of lysate from (1) HepG2 cell line and (2) human lung tissue lysate using ALDH1A1 antibody diluted at 1:1000.
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